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Literature summary extracted from

  • Taguchi, T.; Ogawa, T.; Inoue, S.; Inoue, Y.; Sakamoto, Y.; Korkane, H.; Taniguchi, N.
    Purification and characterization of UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha1-R [GlcNAc to Man]-beta1,4-N-acetylglucosaminyltransferase VI from hen oviduct (2000), J. Biol. Chem., 175, 32598-32602.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.1.201 dithiothreitol is required for stabilization of the purified enzyme Gallus gallus
2.4.1.201 enzyme becomes more stable after elution from a Ni2+-chelating column during purification Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.201 microsome
-
 Gallus gallus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.201 Ca2+ 8.5% activity compared to Mn2+ Gallus gallus
2.4.1.201 Co2+ can substitute partially for Mn2+ Gallus gallus
2.4.1.201 Co2+ 80% activity compared to Mn2+ Gallus gallus
2.4.1.201 Mg2+ can substitute partially for Mn2+ Gallus gallus
2.4.1.201 Mg2+ 60% activity compared to Mn2+ Gallus gallus
2.4.1.201 Mn2+ maximal activity at 5 mM Gallus gallus
2.4.1.201 Mn2+ absolute requirement Gallus gallus
2.4.1.201 additional information requirement for divalent cations, no effect of Zn2+, Fe2+, Cu2+ Gallus gallus
2.4.1.201 Ni2+ can substitute partially for Mn2+ Gallus gallus
2.4.1.201 Ni2+ 27% activity compared to Mn2+ Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.201 60000
-
 x * 60000, reducing SDS-PAGE Gallus gallus
2.4.1.201 72000
-
 x * 72000, non-reducing SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R Gallus gallus biosynthesis of pentaantennary asparagine-linked N-glycans UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.201 Gallus gallus
-
 hen
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.201 glycoprotein
-
 Gallus gallus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.201
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.201 oviduct
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.201 0.032
-
 purified enzyme Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R acceptor substrate specificity Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R R represents the remainder of the N-oligosaccharide core of asparagine-linked glycopeptides, (+/-)-fucose, minimum structure requirement: N-acetyl-D-glucosaminyl-beta-1,6-(N-acetylglucosaminyl-beta-1,2)-alpha-mannosyl-R Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R biantennary oligosaccharides, i.e. Gn T I and II reaction products, are no substrates Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R biosynthesis of pentaantennary asparagine-linked N-glycans Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.201 ? x * 72000, non-reducing SDS-PAGE Gallus gallus
2.4.1.201 ? x * 60000, reducing SDS-PAGE Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.201 37
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.201 7.8
-
-
 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.201 additional information
-
 broad range Gallus gallus